Roche recently announced the launch and immediate availability of the GS GType TET2/CBL/KRAS and the GS GType RUNX1 Primer Sets for comprehensive genetic variation detection in four key human genes using the company's 454 GS Junior
and GS FLX Systems.
The sequence-based assays offera new solution for cancer researchers investigating the human TET2, CBL, KRAS and RUNX1
genes, which are known to be associated with developmental defects, disease progression, and residual disease in a variety of leukemias and myeloid malignancies. The assays enable deep sequencing of PCR amplicons covering key exon regions, and offer superior sensitivity and time to result compared to traditional Sanger capillary sequencing approaches.
Blood cancers such as leukemia and other myeloproliferative disorders cause rapid, abnormal growth of blood cells and are known to consist of a broad spectrum of subtypes. Currently, a variety of techniques are available to characterize leukemia types, including traditional Sanger capillary sequencing, cytogenetics, and cytomorphology, but are expensive, time-consuming and, in some instances, fail to offer the depth of analysis or sensitivity enabled by next-gen sequencing. Using the GS GType TET2/CBL/KRAS and the GS GType RUNX1 Primer Sets with 454 Sequencing Systems, researchers can detect genetic variants far below the Sanger limit of detection.
The assays, which include primer plates, protocols and dedicated analysis software, have been co-developed with and extensively tested at the MLL Munich Leukemia Laboratory in Munich, Germany. The GS GType TET2/CBL/KRAS Primer Set is the result of the International Robustness of Next-Generation Sequencing (IRON) study.